synaptic glutamate Search Results


affinity-purified rabbit antibodies directed against the vesicular glutamate transporters 135402  (Synaptic Systems)
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Synaptic Systems affinity-purified rabbit antibodies directed against the vesicular glutamate transporters 135402
Affinity Purified Rabbit Antibodies Directed Against The Vesicular Glutamate Transporters 135402, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies to vesicular glutamate transporter 1 vglut 1 135304  (Synaptic Systems)
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Synaptic Systems antibodies to vesicular glutamate transporter 1 vglut 1 135304
Antibodies To Vesicular Glutamate Transporter 1 Vglut 1 135304, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dynamics simulations of glutamate diffusion in membrane bound synaptic cleft  (Molecular Dynamics Inc)
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Molecular Dynamics Inc dynamics simulations of glutamate diffusion in membrane bound synaptic cleft
Dynamics Simulations Of Glutamate Diffusion In Membrane Bound Synaptic Cleft, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vesicular glutamate transporter 2 135404 antibody  (Synaptic Systems)
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Synaptic Systems vesicular glutamate transporter 2 135404 antibody
Vesicular Glutamate Transporter 2 135404 Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lrrc8a ko hela cells  (Johns Hopkins HealthCare)
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vesicular glutamate transporter 1 rabbit polyclonal  (Synaptic Systems)
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Synaptic Systems vesicular glutamate transporter 1 rabbit polyclonal
Vesicular Glutamate Transporter 1 Rabbit Polyclonal, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vesicular glutamate transporter 1 rabbit polyclonal - by Bioz Stars, 2026-04
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anti-vesicular glutamate transporter (vglut) 1  (Synaptic Systems)
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Synaptic Systems anti-vesicular glutamate transporter (vglut) 1
Anti Vesicular Glutamate Transporter (Vglut) 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti-vesicular glutamate transporter 1  (Synaptic Systems)
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Synaptic Systems rabbit polyclonal anti-vesicular glutamate transporter 1
Rabbit Polyclonal Anti Vesicular Glutamate Transporter 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal antibody against glutamate vesicular transporter 1  (Synaptic Systems)
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Synaptic Systems rabbit polyclonal antibody against glutamate vesicular transporter 1
Rabbit Polyclonal Antibody Against Glutamate Vesicular Transporter 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit pab against vesicular glutamate transporter 1  (Synaptic Systems)
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Synaptic Systems rabbit pab against vesicular glutamate transporter 1
Antibodies used
Rabbit Pab Against Vesicular Glutamate Transporter 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal anti-vesicular glutamate transporter 1  (Synaptic Systems)
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Synaptic Systems polyclonal anti-vesicular glutamate transporter 1
(A) Fluorescence micrograph depicting a GFP-positive neuron derived from a fate-mapped astroglia, prepared from postnatal cortex of tamoxifen-induced GLAST::CreERT2/Z/EG mice, 14 d after transduction with Neurog2-DsRed. The inset shows control untransduced GFP-positive astroglia. (B) DsRed expression in the same cell indicating forced expression of Neurog2. (C) Step-current injection into the cell shown in (A) and (B) results in repetitive firing of action potentials. (D–E) Fluorescence micrographs depicting another GFP-positive neuron, derived from a fate-mapped astroglia, following Neurog2-induced reprogramming 19 d after transduction. (F) Step-depolarisation at 1 Hz of the neuron shown in (D) and (E) evokes an autaptic response exhibiting a short decay time (90%–10%) typical of glutamatergic synaptic transmission (5.2 ms). The inset shows a single response evoked at 0.05 Hz revealing both an autaptic and a polysynaptic response (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (G–H) Another example of a GFP-positive neuron derived from a fate-mapped astroglia following Neurog2-induced reprogramming 27 DPI. The inset in (H) shows an enlargement of the boxed area revealing a DsRed-positive axon (arrowheads) originating from another DsRed-positive neuron and meandering along the dendrites and the soma of the recorded cell, after the patch pipette had been withdrawn and the cell died. (I) Step-depolarisation of the neuron shown in (G) and (H) evokes a sequence of both autaptic and polysynaptic responses. The black traces show individual autaptic responses observed in isolation when evoked at 1 Hz to eliminate polysynaptic components (the average trace is shown in red). The autapse exhibited a short decay time (90%–10%) typical of glutamatergic synaptic transmission (7.7 ms). The inset shows two individual responses evoked at 0.05 Hz (black and red traces) revealing both autaptic and polysynaptic responses (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (J–K) Expression of the vesicular glutamate <t>transporter</t> <t>1</t> (vGluT1) by fate-mapped postnatal astroglia reprogrammed by Neurog2. (J) Micrograph depicting a GFP and DsRed double-positive neuron derived from a fate-mapped astroglia, 27 d after transduction with a retrovirus encoding Neurog2 and DsRed. Note the DsRed-negative fate-mapped astrocyte. (K) Immunocytochemistry for vGluT1 reveals that the fate-mapped astroglia-derived neuron reprogrammed by Neurog2 exhibits a dense labelling of vGluT1-positive puncta outlining its cell body and dendrites. The insets show higher magnification views of the soma and dendrites illustrating the punctuate staining for vGluT1. Scale bars: J-K: 30 µm.
Polyclonal Anti Vesicular Glutamate Transporter 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti-vesicular glutamate transporter 1/product/Synaptic Systems
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glutamate nmda receptor subunit 1 (glunr1) antibody, mouse monoclonal igg  (Synaptic Systems)
90
Synaptic Systems glutamate nmda receptor subunit 1 (glunr1) antibody, mouse monoclonal igg
(A) Fluorescence micrograph depicting a GFP-positive neuron derived from a fate-mapped astroglia, prepared from postnatal cortex of tamoxifen-induced GLAST::CreERT2/Z/EG mice, 14 d after transduction with Neurog2-DsRed. The inset shows control untransduced GFP-positive astroglia. (B) DsRed expression in the same cell indicating forced expression of Neurog2. (C) Step-current injection into the cell shown in (A) and (B) results in repetitive firing of action potentials. (D–E) Fluorescence micrographs depicting another GFP-positive neuron, derived from a fate-mapped astroglia, following Neurog2-induced reprogramming 19 d after transduction. (F) Step-depolarisation at 1 Hz of the neuron shown in (D) and (E) evokes an autaptic response exhibiting a short decay time (90%–10%) typical of glutamatergic synaptic transmission (5.2 ms). The inset shows a single response evoked at 0.05 Hz revealing both an autaptic and a polysynaptic response (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (G–H) Another example of a GFP-positive neuron derived from a fate-mapped astroglia following Neurog2-induced reprogramming 27 DPI. The inset in (H) shows an enlargement of the boxed area revealing a DsRed-positive axon (arrowheads) originating from another DsRed-positive neuron and meandering along the dendrites and the soma of the recorded cell, after the patch pipette had been withdrawn and the cell died. (I) Step-depolarisation of the neuron shown in (G) and (H) evokes a sequence of both autaptic and polysynaptic responses. The black traces show individual autaptic responses observed in isolation when evoked at 1 Hz to eliminate polysynaptic components (the average trace is shown in red). The autapse exhibited a short decay time (90%–10%) typical of glutamatergic synaptic transmission (7.7 ms). The inset shows two individual responses evoked at 0.05 Hz (black and red traces) revealing both autaptic and polysynaptic responses (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (J–K) Expression of the vesicular glutamate <t>transporter</t> <t>1</t> (vGluT1) by fate-mapped postnatal astroglia reprogrammed by Neurog2. (J) Micrograph depicting a GFP and DsRed double-positive neuron derived from a fate-mapped astroglia, 27 d after transduction with a retrovirus encoding Neurog2 and DsRed. Note the DsRed-negative fate-mapped astrocyte. (K) Immunocytochemistry for vGluT1 reveals that the fate-mapped astroglia-derived neuron reprogrammed by Neurog2 exhibits a dense labelling of vGluT1-positive puncta outlining its cell body and dendrites. The insets show higher magnification views of the soma and dendrites illustrating the punctuate staining for vGluT1. Scale bars: J-K: 30 µm.
Glutamate Nmda Receptor Subunit 1 (Glunr1) Antibody, Mouse Monoclonal Igg, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Antibodies used

Journal: The Journal of comparative neurology

Article Title: Development of cone photoreceptors and their synapses in the human and monkey fovea

doi: 10.1002/cne.24170

Figure Lengend Snippet: Antibodies used

Article Snippet: The rabbit pab against the vesicular glutamate transporter 1 (Synaptic Systems Cat# 135 302, RRID:AB_887877) recognizes a 60-kDa protein in Western blots of rat brain.

Techniques: Recombinant, Binding Assay, Purification

(A) Fluorescence micrograph depicting a GFP-positive neuron derived from a fate-mapped astroglia, prepared from postnatal cortex of tamoxifen-induced GLAST::CreERT2/Z/EG mice, 14 d after transduction with Neurog2-DsRed. The inset shows control untransduced GFP-positive astroglia. (B) DsRed expression in the same cell indicating forced expression of Neurog2. (C) Step-current injection into the cell shown in (A) and (B) results in repetitive firing of action potentials. (D–E) Fluorescence micrographs depicting another GFP-positive neuron, derived from a fate-mapped astroglia, following Neurog2-induced reprogramming 19 d after transduction. (F) Step-depolarisation at 1 Hz of the neuron shown in (D) and (E) evokes an autaptic response exhibiting a short decay time (90%–10%) typical of glutamatergic synaptic transmission (5.2 ms). The inset shows a single response evoked at 0.05 Hz revealing both an autaptic and a polysynaptic response (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (G–H) Another example of a GFP-positive neuron derived from a fate-mapped astroglia following Neurog2-induced reprogramming 27 DPI. The inset in (H) shows an enlargement of the boxed area revealing a DsRed-positive axon (arrowheads) originating from another DsRed-positive neuron and meandering along the dendrites and the soma of the recorded cell, after the patch pipette had been withdrawn and the cell died. (I) Step-depolarisation of the neuron shown in (G) and (H) evokes a sequence of both autaptic and polysynaptic responses. The black traces show individual autaptic responses observed in isolation when evoked at 1 Hz to eliminate polysynaptic components (the average trace is shown in red). The autapse exhibited a short decay time (90%–10%) typical of glutamatergic synaptic transmission (7.7 ms). The inset shows two individual responses evoked at 0.05 Hz (black and red traces) revealing both autaptic and polysynaptic responses (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (J–K) Expression of the vesicular glutamate transporter 1 (vGluT1) by fate-mapped postnatal astroglia reprogrammed by Neurog2. (J) Micrograph depicting a GFP and DsRed double-positive neuron derived from a fate-mapped astroglia, 27 d after transduction with a retrovirus encoding Neurog2 and DsRed. Note the DsRed-negative fate-mapped astrocyte. (K) Immunocytochemistry for vGluT1 reveals that the fate-mapped astroglia-derived neuron reprogrammed by Neurog2 exhibits a dense labelling of vGluT1-positive puncta outlining its cell body and dendrites. The insets show higher magnification views of the soma and dendrites illustrating the punctuate staining for vGluT1. Scale bars: J-K: 30 µm.

Journal: PLoS Biology

Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons

doi: 10.1371/journal.pbio.1000373

Figure Lengend Snippet: (A) Fluorescence micrograph depicting a GFP-positive neuron derived from a fate-mapped astroglia, prepared from postnatal cortex of tamoxifen-induced GLAST::CreERT2/Z/EG mice, 14 d after transduction with Neurog2-DsRed. The inset shows control untransduced GFP-positive astroglia. (B) DsRed expression in the same cell indicating forced expression of Neurog2. (C) Step-current injection into the cell shown in (A) and (B) results in repetitive firing of action potentials. (D–E) Fluorescence micrographs depicting another GFP-positive neuron, derived from a fate-mapped astroglia, following Neurog2-induced reprogramming 19 d after transduction. (F) Step-depolarisation at 1 Hz of the neuron shown in (D) and (E) evokes an autaptic response exhibiting a short decay time (90%–10%) typical of glutamatergic synaptic transmission (5.2 ms). The inset shows a single response evoked at 0.05 Hz revealing both an autaptic and a polysynaptic response (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (G–H) Another example of a GFP-positive neuron derived from a fate-mapped astroglia following Neurog2-induced reprogramming 27 DPI. The inset in (H) shows an enlargement of the boxed area revealing a DsRed-positive axon (arrowheads) originating from another DsRed-positive neuron and meandering along the dendrites and the soma of the recorded cell, after the patch pipette had been withdrawn and the cell died. (I) Step-depolarisation of the neuron shown in (G) and (H) evokes a sequence of both autaptic and polysynaptic responses. The black traces show individual autaptic responses observed in isolation when evoked at 1 Hz to eliminate polysynaptic components (the average trace is shown in red). The autapse exhibited a short decay time (90%–10%) typical of glutamatergic synaptic transmission (7.7 ms). The inset shows two individual responses evoked at 0.05 Hz (black and red traces) revealing both autaptic and polysynaptic responses (asterisk) due to recruitment of other neurons in the cultured network, indicating the excitatory nature of the fate-mapped reprogrammed neuron. (J–K) Expression of the vesicular glutamate transporter 1 (vGluT1) by fate-mapped postnatal astroglia reprogrammed by Neurog2. (J) Micrograph depicting a GFP and DsRed double-positive neuron derived from a fate-mapped astroglia, 27 d after transduction with a retrovirus encoding Neurog2 and DsRed. Note the DsRed-negative fate-mapped astrocyte. (K) Immunocytochemistry for vGluT1 reveals that the fate-mapped astroglia-derived neuron reprogrammed by Neurog2 exhibits a dense labelling of vGluT1-positive puncta outlining its cell body and dendrites. The insets show higher magnification views of the soma and dendrites illustrating the punctuate staining for vGluT1. Scale bars: J-K: 30 µm.

Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).

Techniques: Fluorescence, Derivative Assay, Transduction, Expressing, Injection, Transmission Assay, Cell Culture, Transferring, Sequencing, Isolation, Immunocytochemistry, Staining

Cells from the E12.5 cerebral cortex (white bars), astroglia cultured under adherent conditions (7 d, black bars), and astroglia cultured under neurosphere conditions (7 d, red bars) were compared for the expression of several genes by quantitative RT-PCR. The expression of the mRNAs encoding GFAP (A), S100β (B), Aldh1L1 (C), Glu1 (D), Glt-1 (E), βIII tubulin (F), Neurog2 (G), Emx1 (H), Emx2 (I), and Sox2 (J) was normalized to the respective mRNA amount estimated for E12.5 cerebral cortex tissue and plotted as fold expression.

Journal: PLoS Biology

Article Title: Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional Neurons

doi: 10.1371/journal.pbio.1000373

Figure Lengend Snippet: Cells from the E12.5 cerebral cortex (white bars), astroglia cultured under adherent conditions (7 d, black bars), and astroglia cultured under neurosphere conditions (7 d, red bars) were compared for the expression of several genes by quantitative RT-PCR. The expression of the mRNAs encoding GFAP (A), S100β (B), Aldh1L1 (C), Glu1 (D), Glt-1 (E), βIII tubulin (F), Neurog2 (G), Emx1 (H), Emx2 (I), and Sox2 (J) was normalized to the respective mRNA amount estimated for E12.5 cerebral cortex tissue and plotted as fold expression.

Article Snippet: The following primary antibodies were used: anti-GFP (GFP, chicken, 1∶1000, Aves Labs, GFP-1020 ), polyclonal anti-Glial Fibrillary Acidic Protein (GFAP, rabbit, 1∶4000, DakoCytomation, Z0334 ), polyclonal anti-Red Fluorescent Protein (RFP, rabbit, 1∶500, Chemicon, AB3216 ), polyclonal anti-RFP (rabbit, Rockland, 1∶2000, 600-401-379 ), polyclonal anti-vesicular glutamate transporter 1 (vGluT11, rabbit, 1∶1000, Synaptic Systems, 135302 ), monoclonal anti-Microtubule Associated Protein 2 (MAP2, mouse IgG1, 1∶200, Sigma-Aldrich, M4403 ), monoclonal anti-synapsin 1 (mouse IgG2, 1∶2000, Synaptic Systems, 106001 ), polyclonal anti-vGaT (guinea pig, 1∶200, Synaptic Systems, 131004 ), polyclonal anti-Tbr1 (rabbit, 1∶1000, Millipore, AB9616), polyclonal anti-Tbr2 (rabbit, 1∶500, Millipore, AB9618), monoclonal anti-βIII tubulin (mouse IgG2b, 1∶500, Sigma, T8660), polyclonal anti-GAD1(GAD67) (rabbit, 1∶500, Synaptic Systems, 198013), monoclonal anti-calretinin (mouse IgG1, 1∶200, Millipore, MAB1568), and monoclonal anti-CaM kinase IIα (mouse IgG1, 1∶200, Abcam, ab2725).

Techniques: Cell Culture, Expressing, Quantitative RT-PCR